Polymeric structure of a high-molecular-weight glycoprotein from bovine cervical mucus
نویسندگان
چکیده
منابع مشابه
The isolation of a high-molecular-weight glycoprotein from pig colonic mucus.
The principal components of a variety of mucous secretions have been found to be glycoproteins, many of mol.wt. 500000 or more (Gottschalk, 1972). We have isolated the glycoprotein from pig colonic mucus and examined its structure in order to compare it with that of the well-characterized glycoprotein of the gastric mucus from the same species (Snary & Allen, 1971 ; Allen, 1977). Mucosal scrapi...
متن کاملA 70000-molecular-weight protein isolated from purified pig gastric mucus glycoprotein by reduction of disulphide bridges and its implication in the polymeric structure.
The glycoprotein of pig gastric mucus has been isolated free of non-covalently bound protein as judged by sodium dodecyl sulphate/polyacrylamide-gel electrophoresis and equilibrium density-gradient centrifugation. After reduction with 0.2 M-mercaptoethanol, protein was released from the glycoprotein, which consisted of a major 70000-mol.wt. component and a minor 60000-mol.wt. component. The 700...
متن کاملGlycoprotein from Pig Colonic Mucus
1. A high-molecular-weight glycoprotein constitutes over 80% by weight of the total glycoprotein from water-soluble pig colonic mucus. 2. It was isolated free from nucleic acid and non-covalently bound protein by nuclease digestion followed by equilibrium centrifugation in a CsCl gradient. 3. The glycoprotein has the following composition by weight: fucose 10.4%; glucosamine 23.9%; galactosamin...
متن کاملGlobular structure of human ovulatory cervical mucus.
Human cervical mucus is a heterogeneous mixture of mucin glycoproteins whose relative concentration changes during the ovulatory phases, thereby producing different mucus aggregation structures that can periodically permit the transit of spermatozoa for fertilization. In preovulatory phase, mucus is arranged in compact fiber-like structures where sperm transit is hindered. Previously, through o...
متن کاملCervical mucus: from biochemical structure to clinical implications.
Structure of human cervical mucus plays a pivotal role in female fertility and protection of reproductive health. Investigation of biochemical and biophysical structure of cervical mucus remains a challenge due to complex structural proteins, high content of oligosaccharides and cyclic variability of its structure. We present the current knowledge on chemical and biophysical features of cervica...
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ژورنال
عنوان ژورنال: Biochemical Journal
سال: 1983
ISSN: 0264-6021
DOI: 10.1042/bj2150701